Abstract:The timosaponin in an anti-virus oral liquid was absorbed by great occipital resin to get rid of the disturbance of impurities like sugar,then eluted with 75% alcohol and hydrolyzed by acid to produce sapogenin,whose content was determined with a TCLS-method was sensitive with high specificity and good linear relationship,and the rate of recovery was 97.2%,RSD3.28%.