Abstract:A quantitative method was developed for the determination of icariin in Bushenning tablet by reversed phase HPLC.The number of theoretical plates calculated by Icariin peak was 1697,R=1.8.The standard curve was linear in the concentration range of 4.88~78.02μg/ml,and correlation coefficient was 0.9998.The average recovery and the relative standard deviation were 99.4% and 0.48%,rectivily.