Abstract:Objects:To investigate the influence of herbage containing serum of HuangLianJieDu Tang(HLJDT)on LPS or TNF-α induced neutrophil-endothelium adhesion and its anti-inflammation mechanism.Methods:Based on the model of Human umbilical vein endothelial cell(HUVEC),Rose Bengal Stain was used to study the influence of herbage containing serum of HLJDT on LPS or TNF-α induced neutrophil-endothelium adhesion by pretreated HUVEC4h、12h、24h.Results:The results showed the seram of 1.5h group can decrease cells adhesion by pretreated HUVEC with serum only,but all serum can decrease LPS/INF-α induced cells adhesion by pretreated HUVEC with serum and LPS or TNF-α.Conclusion:The herbage containing serum of HLJDT can decrease the neutrophil-endothelium cells adhesion,inhabiting neutrophil transude from vessel,which may be one of its anti-inflammation mechanisms.
