Abstract:The quantitative determination of isofraxidin in Xiaoyanling tablets was established by HPLC method.HPLC conditions as fellows:column:Intetsil ODS-3(5μm,4.6mm×250mm),mobile phase:CH3CN-0.1% phosphorus acid solution(20∶80).The average recovery and relative standard deviation were 99.4% and 1.32%(n=5)respectively.The method was convenience and reliable.