Determination Method of Anthraquinonoids in Radix et Rhizoma Rhei

Abstract:

Objective:To select the optimum extracted method of anthraquinonoids from radix et rhizoma Rhei by RP-HPLC.Methods:The main factors effecting the total anthraquinonoids,such as hydrolysis time,concentration and volume of acid,extraction times and volume of chloroform were examined by uniform design mthod.The data were calculated by UROS system.Results:The optimum extracted conditions:hydrolysis with 1mol/L H₂SO₄ 10ml for 9min,extracted by 70ml chloroform for two times.Conclusion:This method was validated to show reliable and reproducible.