Protective Effects of Tatal Saponin of Panax Notoginseng and Notoginsenosides on the Hypoxia induced damage of Cultured Vascular Endothelial Cells in Vitro

Abstract:

The model of hypoxia induced damage in cultured vascular endotheliocyte cells was set up in vitro, toxicity was tested by MTT assay, effect was evaluated by classic pharmacodynamic criteria Trypan blue stain, lactate dehydrogenase (LDH) release,and MTT assay. The results showed the mortality rate and LDH release were significantly decreased to compare with the damaged group, (P<0.001), and the cell survival rate was significantly increased (P<0.001)in the protective groups of total saponins of Panax notoginseng and notoginsenoside Rb₁,Rg₁ and Re.