Studies on Pureparation Techniques of Compound Caihutang

Abstract:

The purification conditions by absorption resins were optimized by content determination of saikosaponin A,paeoniforin,hesperidin and glycyrrhizic acid.S type synthetic absorption resin was selected.The optimal purification conditions were:specific input sample amount(ratio of weight of medical raw material to volume of resin,W/V)was one,ltime of water to elute the resin and collecting 4 times of 70% ethanol(V/W) as elution solvent.The yield and contents of paeoniforin and hesperidin of dried extract,purified by the absorption resin used repeatedly for 40 times,was not decreased compared with the new resin.