Effect of Compound 861-contained Serum on CoL-Ⅰ,CoL-Ⅲ Gene Expression of HSC-T6 Cells

Abstract:

To observe the effect of Compound 861-contained(Cpd 861) serum on CoL-Ⅰ、CoL-Ⅲ mRNA expression of HSC-T6 cells in vitro,HSC-T6 cells were exposed in different doses of Cpd 861-contained serum (0.5,1.0,2.0,6.0g/kg) for 48 hours.CoL-Ⅰ、CoL-Ⅲ mRNA levels were measured by quantitative reverse-transcription polymerase chain reaction (RT-PCR).CoL-Ⅰ、CoL-Ⅲ mRNA levels of HSC-T6 cells at different doses of Cpd861-contained serum were lower than those of normal control.Cpd 861-contained serum can decrease CoL-Ⅰ and CoL-Ⅲ mRNA expression of HSC-T6 cells.