Abstract:To study the effects of the BuShenYiZiFang-contained serum (BSYZF) and its subgroups on the differentiation and growth of NG108-15 cells injured with Aβ25-35,and to investigate compatible effects of BSYZF.BSYZF was divided into three subgroups:BUSHEN group,YIQIYAANGXUE group,QUBINGPIAN group (BUSHEN plus YIQYANGXUE,i.e.deleted borneol BSYXF).Four groups (BSYXF and its three subgroups) serum containing correspondent drugs were collected from rats,respectively,and used to culture cells.NG108-15 cells were cultured with Aβ25-35 for establishing cell model of Alzheimer's disease (AD).The cell morphology,cell viability and proliferation,and neurite outgrowth were observed.The results showed that all the herbage-contained serum could protect NG108-15 cells from Aβ25-35 neurotoxicity and the QUBINGPIAN was likely to have the best protective effect.The protective effects of the four groups were in the order:QUBINGPIAN>BSYZF>BUSHEN>YIQAIYANGXUE.
