Determination of Hesperidin in Compound OPG Tablet by HPLC

Abstract:

Obective:To develop a HPLC method for determining Hesperidin in the Compound OPG Tablet.Method:The sample was sonicated for 30 min.The contents were determined by HPLC,with a C18 column a molile phare of methanol-water (41∶59) and detection wavelength at 283nm.Result:Hesperidin has a good linearity in the range of 0.05658～1.13μg,Y=2637.54768X-7.43358,r=0.9999,The average recovery is 104.94% and RSD=2.37%(n=5).Conclusion:The method is sensitive,reliable and speedy.It can used for quality control of Compound OPG Tablet.