Preliminary Study on the Changes of Hypothalamic Proteome Caused by Guizhi Tang in Fever Rats

Abstract:

Aim: To further explore the molecular mechanism of antipyretic action of Guizhi Tang, the differences on the protein expression in hypothalamus of fever rats induced by yeast were analyzed preliminarily. Methods: Both the quantitative and qualitative changes of the protein expression in hypothalamus were compared between the model group and treated group with Guizhi Tang using two-dimensional polyacrylamide gel electrophoresis. Results: There are obvious differences in expression of some proteins with a percentage of 2.3% among distinguishable spots. They changed mostly in volume and rarely in isoelectric point, of which, eight proteins (M_r/pI:28.9 kD/4.47,24.4 kD/6.28,25.4 kD/6.39,25.9 kD/6.39,17.6 kD/7.38,17.2 kD/7.43,24.9 kD/7.39,26.9 kD/7.59) enhanced in abundance, six proteins(M_r/pI:14.3 kD/4.83,28.5 kD/4.39,16.2 kD/4.11,15.3 kD/6.7,30.5 kD/7.09,30.5 kD/7.13)showed lower expression and one (M_r/pI:14.8 kD/5.4) changed in isoelectric point. There was no unmatched protein spot. Conclusions: The changes in expression level and modification of some proteins may contribute to the antipyretic action of Guizhi Tang.