Experimental Study of Shuang Ling Fuzheng Kangai Capsule(SLFKC) on Inhibiting Heps of Mouse

Abstract:

Objective:To study the inhibitory effect and mechanism in Heps of mouse with Shuang Ling Fuzheng Kangai Capsule(SLFKC). Method:Mouse model of the solid tumor and ascitic cancer were established.The inhibitory rate of solid tumor and the life prolongation rate of ascites cancer were observed.The cell cycle and apoptosis of the solid tumor cells were measured by Flow Cytometry(FCM). Results:SLFKC(15 and 30g.kg^-1.d^-1) had significant restraning effect on Heps of mouse and the life of ascites cancer bearing mice was also prolonged. SLFKC could decrease the number of cells in S and G₂-M phase and increase the number of cells in G₀-G₁ phase and the number of apoptosis cells. Conclusion:SLFKC has inhibiting effect on Heps of mouse. The anticancer mechanism was probably related to the synthesis of DNA during S phase,the interference with the speed of cell proliferdtion,and inducement of the apoptosis of cancerous cells.