Effect of Decoction of Rehmanniae on the Expression of Apoptosis of Hippocampal Neurons Model of Alzeimer Disease

Abstract:

Objective:To investigate effects on the expression of apoptosis gene bcl-2、bax and caspase-3 of Decoction of Rehmanniae on cultured hippocampal neurons injury induced by β-amyloid_25～35 protein(A β_25～35).Methods:Cultured hippocampal neurons were divided into 6 groups by using the cerebrospinal fluid with Traditional Chinese medicine normal group、model group、vitamin E group、Low dose of TCM group、moderate dose of TCM group and high dose of TCM group.Six group were respectively added to normal cultured fluid、normal cerebrospinal fluid、VitE cerebrospinal fluid、low dose of TCM cerebrospinal fluid、moderate and high dose of cerebrospinal fluid,hatched 2 hours.The normal group was added to the same dose of cultured fluid and other groups was added to A β_25～35dealed with aging(the final concentration is 10μmol/L).and then six groups were hatched 24 hours together. At last neurous were digested by trypsin,centrifuged,collected and then distilled the total RNA.The expression of bcl-2、bax and caspase-3 by using the methods of RT-PCR and agarose gelelectrophoresis were measured. Results:The group of Decoction of Rehmanniae cerebrospinal fluid can dose dependedly enhance the expression of bcl-2 gene,reduce the expression of bax and caspase-3 gene injured hippocampal neurons.It showed that Decoction of Rehmanniae cerebrospinal fluid had the inhibitory effects on apoptosis of cultured Hippocampal neurons injury induced by A β_25～25.The mechanism may associate that Decoction of Rehmanniae enchanced the ratio of bcl-2/bax.restrained mitochondrial from releasing cytochrome c,controlled the activation of caspase-3.