Abstract:Objectives: To determine the content of chlorogenic acid in Yinqiaojiedu Granuces by HPLC. Method: The analysis was performed on an hypersil C18 column, with a mobile phase of methanol-water-acetic acid-glacial-triethylamine (15:85:1:0.2), the detection wavelength was 254nm. Result: Recovery of chlorogenic acid was 98.12% RSD=0.97%(n=5). Conclusion:This method is simple and reproducible, and it can be used for the quantitative analysis of chlorogenic acid in Yinqiaojiedu Granuces.