Investigation of Attenuating Toxicity Mechanism of Processing for Arisaema erubescens (Ⅰ)

Abstract:

Objective:To investigate attenuating toxicity mechanism of processing for Arisaema erubescens. Method: Toxicity of raw products,processed products,raphides and different extraction site of A. erubescens were compared by acute toxicity test in mice and rabbit conjunctival irritation test;Change condition of special calcium oxalate raphides and impact of related ion from alum on morphological change of raphides before and after processing of A. erubescens were observed by scanning electron microscopy. Result: Acute toxicity test results showed that LD₅₀ of raw products powder group,processed products powder group,raphides group of A. erubescens were 1062,2788,42.53 mg·kg^-1.Results of rabbit conjunctival irritation test showed that raw powder group and raphides group had low and moderate irritation,but other various extracts had no irritation.Electron microscopy showed that ultrastructure of raphides occurred significantly change before and after processing of A. erubescens,and it was destroyed after soaked in KAl (SO₄)₂·12H₂O or AlCl₃ solution for 12 h,but no big change after soaked in Na₂SO₄ or KCl solution,the tip still existed. Conclusion: Raphides from A. erubescens was main material foundation of irritating toxicity,irritation toxicity of raphides from A. erubescens was related with its morphology, mechanism of attenuating toxicity of processed A. erubescens by alum was that Al³⁺ may accelerate destruction of calcium oxalate raphides morphology.