Abstract:Objective:To optimize extraction and purification technology of total triterpene acid from Hedyotis diffusa. Method: With extraction rate of total triterpene acid as index,the amount and concentration of ethanol,reflux time were chosen as factors,extraction technology of total triterpene acid from H. diffusa was optimized by orthogonal test;Resin model was screened by static adsorption and desorption test,purification technology of total triterpene acid was investigated by single factor test. Result: Optimum extraction technology was:reflux extracted 1.5 h with 10-fold 70% ethanol.Optimum purification technology of AB-8 macroporous resin was:sample flow rate 2 BV·h-1,the concentration of sample liquid 0.25 g·L-1, pH 5,the concentration of elution was 80% ethanol,eluting velocity 2 BV·h-1 with pH 7. Conclusion: Optimized technology was simple,stable with good reproduciblity,which was suitable for industrial production of total triterpene acid.
