Abstract:Objective:To establish a method of fingerprint analysis on Yiqing soft capsule by HPLC and provide a reference for inner quality evaluation of Yiqing soft capsule. Method: The separation was performed on Xtimate-C18 chromatographic column with mobile phase consisted of water (contains 0.2% phosphoric acid)-acetonitrile (gradient elution) at a flow rate of 0.6 mL·min-1. Detecting wavelength was set at 280 nm and the column temperature was set at 30 ℃. About fifteen samples of Yiqing soft capsule from five manufacturers were determined and the similarity of each chromatogram was analyzed. The adscription of each peak was by comparing nine standard sample and three single herb preparation with Yiqing soft capsule. Result: HPLC-fingerprint of Yiqing soft capsule was established, which contained 18 common peaks, and eight peaks in the fingerprint were identified. The different batch samples made by the same producer had good similarity, but there were great differences among different manufacturers. There was great variability in the content of anthraquinones in individual crude drugs Rhubarb from different manufacturers and batches, but the main component in coptis root and scutellaria is relatively stable. Conclusion: The method with good reproducibility is accurate and reliable, it can be used in quality control of Yiqing soft capsule.
