RP-HPLC Fingerprint Analysis of Folium Isatidis Pieces from Various Habitats

Abstract:

Objective:RP-HPLC was used to establish a method of fingerprint analysis on Folium Isatidis Pieces. This method can provide a reliable basis for the quality control. Method: Chromatographic column: Inertsil ODS-3 (4.6 mm×250 mm, 5 μm); mobile phase: ACN-0.1％formic acid, gradient elution; flow rate: 1.0 mL·min^-1; detection wavelength: 254 nm; column temperature: 30 ℃. Result: The normal characteristic fingerprint of Folium Isatidis Pieces was established. The result showed that 32 peaks were characteristic including four common peaks and one partially detected peak. Conclusion: With high specificity, precision and good repeatability, the established method provides an effective means for overall quality control of Folium Isatidis Pieces.