Abstract:Objective:A rapid method was established for quantitative determination of peptide based on biuret reaction-enzyme-linked immunosorbent detector, and peptide content in the same geographical origin of pre-and-post-processed Trionycis Carapax was analyzed. Method: The developer was 0.02 g·mL-1 sodium potassium tartrate solution (50 μL), 5% NaOH (20 μL), 1% CuSO4·5H2O (50 μL). The wavelength of detection was at 580 nm. Result: The calibration curves were linear in the ranges of 0.00-5.00 g·L-1 for septa-peptide from Trionycis Carapax. Peptide content in vinegar-processed samples was 6.99%, while the same content in crud samples was 1.04%. Conclusion: Peptide content in vinegar-processed samples was significantly higher than the one in crud samples. The content of Trionycis Carapax is increased greatly after vinegar by decoction.
