Determination of Microwave-Assisted Extraction of Chinese Cut Crude Drugs(6) Comparison of the Determination of Linarin from Chrysanthemum indicum Between Microwave-Assisted Extraction and Chinese Pharmacopeia Method

Abstract:

Objective:To establish a microwave-assisted extraction method for the extraction of the linarin from Chrysanthemun indicum. Comparison of the determination of linarin from C. indicum between microwave-assisted extraction and Chinese pharmacopeia method was made. Method: Dikma Diamonsil C₁₈ column(4.6 mm×150 mm,5 μm)was used. The mobile phase cousisted of methanol-water-acetic acid (26∶23∶1).The temperature of column was kept at 30 ℃. The flow rate was 1.0 mL·min^-1 and the detection wave-length was at 334 nm. Result: Microwave extractive time was 15 min,the temperature was 100 ℃,the extraction solvent was 50% ethanol. The calibration curve was in good linearity within the range from 0.04 to 1.2 μg(r=0.999 9). The average recovery was 100.13%(n=6). Conclusion: The method is simple,rapid,as well as precise and reliable,and can be used for the determination of linarin from C. indicum.