Abstract:Objective:To discuss detection method of the qualitative and quantitative of Erigeron canawdensis. Method: TLC was used to determine quercetin of E. canawdensis. The content of quercetin was determined by HPLC. Result: n-Hexane-butyl acetate-formic (6∶5∶1) is used as TLC deployment system. It may clearly detect quercetin; Shimadzu C18 (4.6 mm×250 mm, 5 μm) column was used in HPLC with mobile phase methanol-0.4% phosphoric acid (47∶53). The detection wavelength was set at 257 nm and the flow rate was 1 mL·min-1; the column temperature was at 30 ℃.The linear range of quercetin were respectively 0.504-1.134 μg, and their average recovery were respectively 97.97%,RSD were respectively 1.2%. Conclusion: This method is used as detection method of the qualitative and quantitative of E. canawdensis.
