Abstract:Objective:To establish the determination method and variation of medicinal parts of Juglans mandshurica. Method: Extract the total flavone from Qinglongyi by 75% ethanol, take the aluminum nitrate-nitrite colorimetric, in addition to the pigment by AB-8 macroporous resin and establish the determination method. Result: The content of total flavone in five different acquisition time was 3.25,3.49,3.90,3.98,3.96 mg·g-1. Conclusion: Before the canicular days,the content of total flavones was gradually increased; after the canicular days, the content of total flavones was significantly growth to flatten, the content of total flavones of medicinal parts of J. mandshurica was leaf>bark> stem.