HPLC Simultaneous Determination of Three Active Components in Ganershu Pellets

Abstract:

Objective:To establish a method of determining quercetin, kaempferide and isorhamnetin in Ganershu pellets by HPLC. Method: An Hedera ODS-2 column(4.6 mm×200 mm,5 μm)was used at 30 ℃ with the mobile phase of 0.4% phosphate (A) and methanol (B)by gradient elution.The detection wavelength was set at 360 nm and the flow rate was 1.0 mL·min^-1. Result: The quercetin, kaempferide and isorhamnetin had good linear relationship within the ranges of 0.042 8-0.42 8 μg(r=0.999 8), 0.003 49-0.034 9 μg (r=0.999 1),0.041 8-0.418 μg(r=0.999 2).The average recoveries were 99.88%,99.44% and 97.50% with RSD 0.77%(n=6),1.2%(n=6),1.5%(n=6) respectively. Conclusion: The method was accurate and reproducibility was good. The method can be used for the quality control of Ganershu pellets.