Abstract:Objective:To establish the quality control methods for Triticum aestivum. Method: The pharmacognostical methods were employed for the morphological and histological characters of Triticum aestivum. TLC was used to identify 5-heneicosyl resorcinol and the content of 5-heneicosyl resorcinol was determined by HPLC. Result: The morphological and histological characters of Triticum aestivum were observed. The spots were observed in the TLC with high characterisitic and good repeatability. Under the selected HPLC method, 5-heneicosyl resorcinol was well separated with others. The linear range of 5-heneicosyl resorcinol was 129.5-2 590 ng. Y=5 056.5X+201.5(r=0.999 9).The average recovery of 5-heneicosyl resorcinol was 101.26%, and the RSD was 1.89%. Conclusion: The established method is simple, feasible, reproducible and accurate. It is suitable for identification and evaluation of the quality, and can be used as the quality control standard of Triticum aestivum.
