Quality Standard of Biyanling Pill

Abstract:

Objective:To establish a method for quality control of Biyanling Pill. Method: Cocklebur fruit,Flos magnoliae liliflorae, Mentha haplocalyx and Asarum were identified by TLC, and the content of baicalin in pills was determined by HPLC with C₁₈ column and the mobile phase consisted of methanol-water-phosphoric acid solution (47∶53∶0.2). Detection wavelength was set at 278 nm. Result: Cocklebur fruit, Flos magnoliae liliflorae, Mentha haplocalyx and Asarum could be identified by TLC. The calibration curves of baicalin was in a good linearity in the range of 0.125 6-2.198 μg (r=0.999 9). The average recovery rate was 98.05% with RSD of 1.66% (n=6). Conclusion: The method is simple, accurate and can be applied to the quality control of Biyanling Pill.