Determination of Geniposide in Yinzhi Oral Solution by HPLC

Abstract:

Objective:To establish an HPLC method for determination of geniposide in Yinzhi oral solution. Method: The Agilent Eclipse XDB-C₁₈ (4.6 mm × 150 mm, 5 μm) was used with the mobile phase consisted of methanol-water (25∶75); the flow rate was 1.0 mL·min^-1, the detection wavelength was at 238 nm, and the column temperature was at 30 ℃. Result: A good linear correlation of geniposide was observed within the range of 0.010 20-0.408 0 μg. The average recovery was 99.35% with RSD 1.90% (n=6). Conclusion: The method is rapid, simple and accurate. It can be used for the determination of geniposide in Yinzhi oral solution.