Abstract:Objective:To determine the content of total flavonoids in several different sources of Gaoziban. Method: Using rutin as a reference substance, the content of total flavonoids in Gaoziban of Asperugo procumbens from Hetian, Cele, and Yutian, Anchusa italica,Borage officinales, and Cynoglossum officinales were determined at 510 nm by UV spectrophotometry colored with sodium nitrite-aluminum nitrate-sodium hydroxide. Result: Content of total flavonoids in three Gaozibans of Anchusa italica, Borage officinales, Cynoglossum officinales were 3.85, 3.72, 2.35 mg·g-1; and three Gaozibans of A. procumbens from Hetian, Cele and Yutian were 3.14, 4.73, 3.67 mg·g-1 respectively, among which the content of total flavonoids in Gaoziban was highest in A. procumbents from Cele and lowest in C. officinales. Conclusion: The method was accurate, reliable, repeatable, and could be used to determine the content of total flavonoids in Gaoziban. The content of total flavonoids is not much different from A. italica, B. officinales and A. procumbens.
