Determination of Salvianolic Acid B in Lemai Tablets by HPLC

Abstract:

Objective:To establish a method for determination of salvianolic acid B in Lemai tablets by HPLC. Method: A Waters SymmetryShield RP18 column (3.9 mm×150 mm,5 μm) was used with the mobile phase of acetonitrile-methnol-1.7% formic acid (4∶27∶69) at the detection wavelength of 286 nm. Result: The calibration curve was linear in the range of 0.20-2.5 μg (r=0.999 9). The average recovery was 100.2% (n=6, RSD 2.0%). Conclusion: The method is convenient, quick, accurate and suitable for the determination of salvianolic acid B in Lemai tablets.