Determination of Mangiferin in Yunfu Qinghuo Pills by HPLC

Abstract:

Objective:To establish a method for the determination of mangiferin in Yunfu Qinghuo pills. Method: Using HPLC,chromatographic conditions:the chromatographic system consisted of Thermo BDS HYPERSIL C₁₈ column,using acetonitrile-0.2% acetic acid glacial (15∶85) as mobile phase.The content was detected at a wavelength of 258 nm,the flow rate was 1.0 mL·min^-1. Result: Mangiferin was linear in the range of 0.053 50-0.374 5 μg(r=0.999 8),the average recovery was 99.22% and RSD was 1.15%(n=6). Conolusion: The method is simple, accurate, reproducible and can be used for the determination of the content of mangiferin in Yunfu Qinghuo Pills.