Determination of Quercetin and Kaempferol in Baihua Sheshecao Dispensing Granule by HPLC

Abstract:

Objective:To establish an HPLC method for determination of quercetin and kaempferol in Baihua Sheshecao dispensing granule. Method: Orthogonal experiments were used to optimize extraction technology. The samples were separated on a Hypersil ODS C₁₈ (4.6 mm×250 mm, 5 μm) column which was eluted with methanol-0.2% phosphoric acid solution (45∶55) with detective wavelength at 360 nm and flow rate at 1 mL·min^-1, column temperature at 30 ℃. Result: The method had good linear relationship within the range of 0.09-0.72 μg (r=0.999 9) for quercetin and 0.034 3-0.274 4 μg (r=0.999 9) for kaempferol. The average recovery rate were 100.80% (RSD 1.79%) and 101.75% (RSD 1.76%). Conclusion: The method is simple, rapid and reliable which can be used to determine quercetin and kaempferol in Baihuashe Shecao dispensing granule.