Abstract:Objective:Use flame atomic absorption spectrometric method for the determination of trace elements content in Dioscoreae Rhizoma. Method: Six Dioscoreae Rhizoma samples of six different sources were purchased and the mixed solution of HNO3 and HClO4 (4∶1)was used to digest the sample of Dioscoreae Rhizoma. The content of Mn, Fe, Cu, Zn, Mg, in the sample solution was determined by FAAS. Result: Dioscoreae Rhizoma medicinal materials contain trace elements, its content from high to low was Mg>Fe>Cu>Zn>Mn. The recovery rate of this method was 95.50%-103.50%. Conclusion: The method is sensitive, rapid, stable, and can be used for determination of trace elements in Dioscoreae Rhizoma.