Abstract:Objective:Through to the studies on fingerprint of cerebrospinal fluid containing medicine of Suanzaoren tang(SZRT), provides the methodology basis for research of efficacy material base of SZRT how to play calm hypnotic effect. Method: By HPLC, with the way of gradient elution, in 50 minutes, established the fingerprint containing water-soluble ingredients and fat soluble constituents. Result: Through screening the chromatographic conditions, such as the gradient of elution and detected wavelength, established the fingerprint that can separate from each other the chemical composition of cerebrospinal fluid containing medicine of SZRT. And through the belonging analysis, preliminary identified the inherent composition of cerebrospinal fluid containing medicine, the drug-induced composition and metabolic product. Conclusion: This method can be used as an effective means to research efficacy material base of SZRT.
