Abstract:Objective:To investigate the molecular mechanism of the anti-influenza A virus effects of epigallocatechin gallate(EGCG) derived from green tea in vitro. Method: The cell viability of EGCG was determined by using MTT assay. And virus inhibitory rate was evaluated based on the observation of cytopathic effect (CPE), hemagglutination assay and Real-time Quantitative PCR (QPCR). The mechanisms of action for the observed sensitivity of influenza A to EGCG at the molecular level was assessd by confocal microscope and flow cytometry. Result: Exposing Madin-Darby canine kidney (MDCK) cells with 20 mg·L-1 EGCG could inhibit influenza A replication in a time-dependent manner, probably by interrupting postbinding step. EGCG treatment could mitigate oxidative stress in influenza-infected cells and reduce the apoptosis induced by influenza infection. Conclusion: EGCG inhibits oxidative stress and apoptosis induced by influenza virus infection.
