Abstract:Objective:To observe the effect on the flavoniod of Huanglian Jiedu decoction, and discuss its material base in reversing multiple-drug resistance(MDR) of cancer so as to provide the theory basis for Huanglian Jiedu decoction in clinical practices. Method: MTT assay was adopted to test the sensitivity of baicalin and geniposide to adriamycin(ADM) of K562/ADM, the inhibitory rate was calculated, the value of 50% inhibition concentration(IC50) and the multiple of the reversal of drug resistance as well as the change of the concentration of the intracellular ADM were assayed by using ADM K562/ADM of human chronic myelogenous leukemia(CML) erythroleukemia K562 as cell line. Result: Both baicalin and geniposide could partly reverse K562/ADM cell. The value of IC50 of baicalin and geniposide was 5.06 mg·L-1and 6.74 mg·L-1 respectively.The multiple of the reversal of drug resistance of baicalin and geniposide was 1.95 times and 1.46 times.The fluorescence intensity of the intracellular K562/ADM was higher than the control group, and the fluorescence intensity in the group of baicalin rised to 3.6%, The fluorescence intensity of the group of geniposide rise to 1.7%. Conclusion: The material base of the Huanglian Jiedu decoction in reversing MDR of cancer is probably related to its components baicalin and geniposide.
