Abstract:Objective:To investigate radiosensitization and mechanism of Chinese medicine Jiawei Shengmai San on nasopharyngeal carcinoma. Method: The SD rats swimed in Glass tank for 10 minute in 30 days, at the same time,subcutaneous injection of dinitrosopiperazine(DNP) was performed,the dose was 0.5 mg every time,every 3 days the injection was carried out once for 90 days. Then,nasopharyngeal carcinoma models with Qi deficient status was produced.The samples were divided into control group,drug group,radiotherapy group,drug+radiotherapy group.The radiosensitization effect was measured by measuring tumor volume and weight,and multidrug resistance 1 gene(MDR1) expression was detected by quantitative PCR and western-blot. Result: In the control group tumor weight was (1 230.0±106.8) mg,in the radiotherapy group being (447.2±121.9) mg. Compared with the control group,the ratio of tumor inhibition was 53.25%, there was a significant difference (P<0.05). In the drug+radiotherapy group tumor weight was (259.9±64.7) mg,the ratio of tumor inhibition was 72.81%, there also was a significant difference (P<0.05). The Relative expression ratio, in the drug group was 1.21,the radiotherapy group was 1.85,drug+radiotherapy group was 1.34. Conclusion: Jiawei Shengmai San can enhance the sensitivity of radiotherapy to the nasopharyngeal carcinoma,and its mechanisms may be related to regulating MDR1 gene expression.
