Influence of Matrine on Cell Action Potential in Guinea Pig Papillary Muscle

Abstract:

Objective:To observe influence of matrine on cell action potential in guinea pig papillary muscles thus to clarify its antiarrhythmic mechanism. Method: Guinea pigs were divided into control group, matrine group(10,50,100 μmol·L^-1),aconitine group 1 μmol·L^-1, aconitine 1 μmol·L^-1＋matrine 50 μmol·L^-1group, aconitine 1 μmol·L^-1+matrine 100 μmol·L^-1 group. Conventional microelectrode technique was used to record action potential duration(APD), action potential amplitude (APA), 50 % and 90 % of APD repolarization(APD₅₀ and APD₉₀). Result: Matrine could prolong moderately APD₅₀ and APD₉₀ in a dose-dependent manner(10,50,100 μmol·L^-1)and reduce APA. Aconitine could excessively prolong APD (vs control group, P<0.05), APD₅₀ and APD₉₀. Matrine(100 μmol·L^-1)could remedy for excessive elonggation of aconitine on APD,APD₅₀ and APD₉₀ (vs aconitine group, P<0.05 or P<0.01). Matrine(100 μmol·L^-1)also could reduce APA(vs aconitine group, P<0.05). Conclusion: Matrine could remedy for excessive elonggation of aconitine on APD,APD₅₀ and APD₉₀ and reduce APA of aconitine-induced arrhythmia, which could maitain action potential in a normal range, and was probably one of matrine antiarrhythmic mechanisms.