Abstract:Objective:To observe the anti-inflammatory effects of Viola yedoensis, and analyze the mechanism. Method: The models of ear edema induced by xylene and paw edema induced by carrageenan in mice were used to observe the anti-inflammation effects, the spectrophotometric method and the enzyme-linked immunosorbent assay (ELISA) were used separately to measure the content of prostaglandin E2 (PGE2) in inflammatory tissue and the content of tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β) in serum. Result: Compared with the model control group, the aqueous extraction and alcohol extraction of V. yedoensis (9.0, 3.0 g·kg-1) could significantly inhibit the inflammation caused by xylene or carrageenan (P<0.05-P<0.01), and decreased the content of PGE2 in inflammatory tissue and the content of TNF-α, IL-1β in serum of the mice. induced by carrageenan. There were significant difference between the high dose group (9.0 g·kg-1) and the model control group (P<0.01). Conclusion: The aqueous extraction and alcohol extraction of V. yedoensis have significant anti-inflammation effects, which may be related to the inhibition of the generation of TNF-α, IL-1β and PGE2.
