Abstract:Objective:To observe the effects of the separate application and time-dependent combination of Ziyin prescription(Liuwei Dihuang Pill, LWDHP) and Yiyang prescription (Jingui Shenqi Pill, JGSQP) on hypothalamus-pituitary-adrenal-thymus(HPAT) axis of kidney deficient aging model rats. Method: The rats were randomly divided into six groups. In addition to the normal group, other groups were injected 5%D-galactose solution 100 mg·kg-1 combined with glucocorticoid dexamethasone solution 0.5 mg·kg-1 to establish the model for six weeks, after establishing the model two weeks, each medication group was given LWDHP, JGSQP, LWDHP(morning use)combined with JGSQP (night use), JGSQP (morning use) combined with LWDHP(night use),1.16 g·kg-1 for four weeks, then the content of serum cortisol(COR),plasma adrenocorticotropic hormone(ACTH), corticotropin-releasing hormone(CRH),and CD4+,CD8+were detected. Result: Compared with natural group, serum COR of kidney deficient aging model group significantly decreased(P<0.05), plasma ACTH, CRH significantly increased(P<0.05),the level of CD8+ significantly decreased(P<0.01), CD4+/CD8+ significantly increased(P<0.01). Compared with kidney deficient aging model group, COR of Ziyin combined with Yiyang group(ZY group) significantly increased(P<0.01), ACTH of Yiyang group, Yiyang combined with Ziyin group(YZ group) significantly decreased(P<0.05), CRH of Ziyin group , ZY group, YZ group significantly decreased(P<0.05 or P<0.01),the level of CD8+, CD4+/CD8+ have no significant differences, the level of CD4+ have no significant differences between each group. Conclusion: The effects of separate application and time-dependent combination of Ziyin prescription and Yiyang prescription on HPA axis of kidney deficient aging model rats are different, ZY prescription have the best effect, but these prescriptions have no effect on immunity.
