Abstract:Objective:To investigate anti-inflammatory and antioxidant function of total flavones of Bidens bipinnata L (TFB) on lipoplysaccharide(LPS)-induced acute lung injury(ALI) and the mechanism. Method: One hundred and twenty SD rats were randomly divided into six groups:control group(physiological saline,ip),model group, dexamethasone group(5 mg·kg-1,ip), TFB treatment group(100,150,200 mg·kg-1,ip), twenty rats in each group. The rat ALI model was induced by LPS,5 mg·kg-1,ip. Six hours after modeling, all rats were sacrificed, protein content, white blood cell in bronchoalveolar lavage fluid(BALF), expression of NF-κB p65, and level of tumor necrosis factor(TNF-α), interleukin-6(IL-6),interleukin-10(IL-10)superoxide dismutase(SOD),malondialdehyde(MDA) and the activity of myeloperoxidase(MPO) in lung were measured. Result: Compared with control group, content of WBC and the protein in BALF was higher(P＜0.05-P<0.01), compared with model group, NF-κB p65 expression level was(38.46±4.56)%,(31.39±4.18)% (in TFB high and medium dose group, respectively), the content of MDA was(1.73±0.19),(1.46±0.15)nmol·mg-1,and the content of TNF-α was(259.33±37.48),(211.46±32.69)μg·L-1,and the content of IL-6 was(287.46±100.18),(223.55±93.49)ng·L-1,and the activitiy of MPO was (2.69±0.57),(2.43±0.38)U·g-1, respectevly.NF-κB p65 expression level and the content of MDA, TNF-α,IL-6 and the activities of MPO in lung were significantly lower(P＜0.05-P<0.01).On the other hand, the level of IL-10 was(16.73±3.87),(17.28±3.58)μg·L-1, the content of SOD was (69.46±9.84),(73.24±8.39)U·mg-1,the content of IL-10 and SOD was increased significantly(P＜0.05-P<0.01). Conclusion: TFB can reduce pulmonary vascular permeability of LPS induced acute lung injury, reduce the inflammatory exudation and oxidative stress damage.
