Abstract:To explore the effect of total flavonoid on cerebral ischemia reperfusion injury in rats. Method: The middle cerebral artery occlusion (MCAO) was used to induce focal cerebral ischemia reperfusion injury model in rats, and the normal Wistar rats were used as control group. After Caragana microphylla Lam total flavonoid (CMF) at dose of 25,50,100 g·kg-1 and aspirin 12.5 mg·kg-1 was orally given for 4 weeks, the hemorrheologic parameters including blood viscosity(η), platelet aggregation rate (PAgT), erythrocyte aggregation index(EAI), rigidity index (IR), erythrocyte deformed index (DI), were observed. The contents of superoxide dismutase (SOD) and malonyldialdehyde (MDA) were determined. Result: In terms of the change of hemorheology [η(mPa·s), PAgT(%), EAI, IR, DI], positive control group (1.71±0.08) mPa·s,(56.49±3.74)%,(8.83±0.59),(3.94±0.22),(0.69±0.08), CMF medium group (1.72±0.11) mPa·s,(57.67±4.31)%,(8.30±0.92),(3.99±0.22),(0.73±0.06) and CMF high dose group (1.57±0.10) mPa·s,(55.59±4.58)%,(7.83±0.58),(3.65±0.17),(0.70±0.07) were obviously reduced in η, PAgT, EAI, IR. And they were decreased in DI. Through Comparation of different groups in contents of SOD and MDA, CMF low (55.45±11.67)U·mL-1,(1.38±0.96)nmol·mL-1, medium (60.23±13.59)U·mL-1,(1.17±0.44)nmol·mL-1, high dose group (64.58±9.36)U·mL-1,(1.08±0.42)nmol·mL-1 increased the bioactivity of SOD and decreased the content of MDA. Conclusion: The CMF has a protective effect on cerebral ischemia reperfusion injury in rats; it might be relevant to the role of anti-oxidative stress and decreased blood viscosity.
