Abstract:Objective:To study the influence of Bushen Huazhuo Fang (BHF) on improving leptin and insulin resistance in rats with nonalcoholic fatty liver, and to explore its mechanism. Method: Forty-five Wistar rats were randomly divided into blank group, model group, silybin capsules(19 g·kg-1) group and BHF group. The blank group was fed with normal diet, model group, silybin capsules and BHF group were fed with the high fat diet. Silybin capsules and the BHF group were fed with the corresponding drug. After 12 weeks, rats were sacrificed, the rat liver index, Lee’s index, insulin resistance index(IRI), insulin sensitivity index(ISI) were calculated. Liver chemical parameters, blood lipids, blood glucose, insulin(INS), leptin(LEP) and tumor necrosis factor and liver Pathological investigation were carried out and evaluated. Result: BHF and silybin could significantly improve the alanine transaminase(ALT), aspartate transaminase(AST), LEP (P<0.01); BHF was better than silybin for improving INS, IRI, ISI (P<0.05 or P<0.01), Silybin had no significant effect on improving INS and IRI. Silybin and BHF groups were better than the model group in fat staining, glycogen staining (P<0.05 or P<0.01). Conclusion: The therapeutic effect of BHF may come from protecting liver, regulating blood lipids, improving LEP and IRI.
