Study of Ampelopsin on Apoptosis in Human Gastric Cancer Cells

Abstract:

Objective:To study ampelopsin on apoptosis in human gastric cancer cells (SGC-7901). Method: Cell proliferation inhibition rate was measured by MTT,The situation in different apoptosis phase was evaluated by flow cytometry (Annexin-V/PI double staining). Result: Ampelopsin could significantly promote early apoptosis in SGC-7901 after 48 h. Ampelopsin of 50 and 75 mg·L^-1 induced apoptosis of (29.03±6.21)%, and (43.43±1.00)% with a dose-dependent relationship, significantly higher than that in the control group (11.83±4.67)% (P<0.05,P<0.01,P<0.01). Cells in G₁ phase was (52.07±4.41)%, (57.07±2.70)%, (58.43±2.66)%, significantly higher than (42.00±3.04)% in control group (P<0.05, P<0.01,P<0.01). Conclusion: Ampelopsin could induce the SGC-7901cell apoptosis and block the cells in G₁ phase.