Optimization of Extraction Technology of Effective Parts in Artemisia annua and Evaluation of Its Repellent Effect

Abstract:

Objective: To optimize extraction technology of effective parts in Artemisia annua and evaluate its repellent effect. Method: Extraction method of repellent effective parts in A. annua was screened by single factor test;With yield of volatile oil as index,effects of time,temperature and pressure on extraction technology was investigated by orthogonal test.Repellent effect of the extract was evaluated with effective protection rate as index. Result: Supercritical CO₂ extraction technology was adopted,optimum technology was as following:temperature 60 ℃,pressure 30 MPa,extraction time 2 h,yield of volatile oil approximately 0.35%.Effective protection rate of these prepared extract of A annua (concentration>0.05 g·mL^-1) reached 99% in 2 hours. Conclusion: This optimized technology was reasonable and stable and its extract had well repellent effect.