Optimization of Extraction Technology for Total Flavonoids from Camellia oleifera Seeds by Response Surface Methodolgy

Abstract:

Objective: To optimize extraction technology of total flavonoids from Camellia oleifera seeds. Method: With extraction temperature,extraction time,ethanol concentration and solid-liquid ratio as independent variables,Box-Behnken central composite design was used to establish mathematical model,response surface and contour were made with yield of total flavonoids as response value,response surface methodology was adopted to optimize extraction technology of total flavonoids from C. oleifera seeds. Result: Optimum extraction technology conditions were as followings:extraction temperature 66 ℃,ethanol concentration 70%,extracting time 1.3 h,solid-liquid ratio of 1:22.Under these conditions,yield of total flavonoids from C. oleifera seeds was 1.87%,which was close to the theoretical value of 1.89%. Conclusion: This optimized technology was stable and reliable,and it could provide reference for rational utilization of resources of C. oleifera.