Optimization of Extraction Process of Anti-tumor Active Ingredient in Clematis manshurica Rupr

Abstract:

Objective: To establish extraction technology for anti-tumor active ingredient of W2 [3-O-α-L-pyran rhamnose-(1→6)-β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-(1→4)-β-D-ribopyranose-(1→3)-α-L-pyran rhamnose-(1→2)-α-L-arabinopyranosyl oleanolic acid saponins] from Clematis manshurica Rupr. Method: With the content of W2 as index,which was determined by HPLC,effects of extraction time,extraction solvent,liquid-solid ratio and extraction times on extraction technology were investigated by single factor test and orthogonal test,then to determine optimum technology of W2.Result: Optimum extraction process of W2 was as following:reflux extracted 2 times with 12 times the amount of 30% ethanol,2.5 hours per time.Conclusion: Optimized technology was reasonable,stable and feasible,which could provide a experimental basis for the follow-up research of C. manshurica Rupr.