Investigation of UPLC Fingerprint for Kangfuxin Liquid

Abstract:

Objective: To establish an UPLC characteristic chromatographic profile analysis method for Kangfuxin liquid,and provide basis for rapid evaluating quality of it. Method: Taking ZORBAX RRHD Eclipse plus C₁₈ column (2.1 mm×100 mm,1.8 μm),with methanol-0.05% acetic acid solution as mobile phase,detection wavelength 256 nm. Fifteen batches of Kangfuxin liquid from different manufacturers were investigated,fingerprint was analysed by 2004A edition of chromatographic fingerprint similarity evaluation system and cluster analysis software. Result: There were 10 common peaks in 11 batches of Kangfuxin liquid from Kelun pharmaceutical and 3 batches from Haoyisheng Panxi pharmaceutical,the peaks similarity was more than 0.990;However,only 8 common peaks in these productions from Inner Mongolia Jingxin pharmaceutical, the similarity was lower than 0.900 by comparing with the control fingerprint.Five common peaks were identified in the fingerprint. Conclusion: This established method was rapid and effective,it could be useful for quality evaluation of Kangfuxin liquid.