Content Determination of Sodium Benzoate and Acesulfame in Ibuprofen Oral Solution by HPLC

Abstract:

Objective: To establish a HPLC method for simultaneously determining the content of sodium benzoate and acesulfame in ibuprofen oral solution. Method: The content of sodium benzoate and acesulfame was determined by HPLC,chromatographic conditions were as follows:J'sphere ODS-H80 column(4.6 mm×250 mm,4.0 μm),the mobile phase of methanol-0.02 mol·L^-1 ammonium acetate (15:85),column temperature 30 ℃,the flow rate of 1.0 mL·min^-1,detection wavelength 230 nm,the injection volume 10 μL. Result: Acesulfame showed a good linear relationship at the range of 15.75-110.25 μg, the average recovery of it was 100.51% with RSD 0.79%.Sodium benzoate showed a good linear relationship at the range of 7.8-47.8 μg,the average recovery was 100.42% with RSD 1.56%. Conclusion: This method was sensitive,simple and reproducible,it could provide a reference for determining the content of sodium benzoate and acesulfame in ibuprofen oral solution.