UPLC Fingerprints of Qili Qiangxin Capsule

Abstract:

Objective: To develop a ultra performance liquid chromatography (UP-LC) method for fingerprints of Qili Qiangxin Capsule. Method: The Waters Acquity UPLC BEH C₁₈ (2.1 mm×100 mm, 1.7 μm) column was used. Acetonitrile and water containing 0.15% phosphoric acid were used as mobile phase of UPLC with gradient elution. The detection wavelength was set at 203 nm. Flow rate was set at 0.5 mL·min^-1 and column temperature was maintained at 40 ℃. Result: UPLC fingerprint was established with 36 common peaks, 8 of 36 common peaks were identified, and the similar degrees of the UPLC fingerprints of Qili Qiangxin capsule from 10 batches were between 0.979 and 1.000. Conclusion: The method of fingerprints has acceptable precision, reproducibility and stability, which can be used as a new means for the quality control of Qili Qiangxin capsule.