Qualitative and Quantitative Determination of Antidiabetic Drugs in Wentangan Capsule by UPLC-MS

Abstract:

Objective: To establish an UPLC-MS method for determination of antidiabetic drugs in Wentangan capsule. Method: Ultra performance liquid chromatography tandem quadrupole mass spectrometer was adopted, LC condition: A agela Venusil-C₁₈ (2.1 mm×50 mm,3 μm) column was used with the isocratic elution of acetonitrile and 0.01 mol·L^-1 ammonium acetate at a flow rate of 0.2 mL·min^-1. MS condition: adopted electrospray ionization (ESI),and multiple reaction monitoring (MRM); monitoring by ion-pairs: repaglinide (453.4→86.0,453.4→230.3), glimepiride (491.4→126.2,491.4→352.3), glibenclamide (494.2→168.5,494.2→368.9), pioglitazone (357.2→119.0, 357.2→134.1), gliclazide (342.2→90.9, 324.2→109.9), glipizide (446.2→92.6, 446.2→102.6), phenethylbiguanide (206.2→59.7, 206.2→104.6), dimethyl biguanide (130.1→60.0, 130.1→71.0). The retention time and relative intensities of the detected qualitative ion pairs using for qualitative, integrating the signals of the detected quantitative ion pairs using for quantitative. Result: The eight kinds of antidiabetic drugs had good linearity with correlation coefficient>0.999, ranging from 4.056-129.792, 13.912-125.184, 3.92-125.44, 3.944-126.208, 4.056-129.792, 4.232-135.424, 4.072-130.304 and 4.264, 136.448 ng·L^-1 respectively. The average recoveries were 98.18-100.95%. Repeatability experiments showed that RSD values of content were less than 2.95%. Conclusion: This method is reliable, simple and rapid with qualitative and quantitative accuracy, can be used for determination of antidiabetic drugs illegally added in Wentangan capsule.