Comparative Study on the Component of Protein, Vitamin C, Vitamin E and Volatile Oil and SOD Activity of Fresh and Dried American Ginseng

Abstract:

Objective: To compare the component of protein, vitamin C, vitamin E and volatile oil and superoside dismutase(SOD) activity of fresh and dried American ginseng and provide reference for reasonable application of American ginseng. Method: SDS-PAGE electrophoresis was carried out for protein analysis; UV spectrophotometry was used for determination of vitamin C and vitamin E; GC-MS was used for the analysis of volatile oil; SOD activity assay was detected with the methods of pyrogallol autoxidation and the data were analyzed by t-test. Result: The electrophoresis bands between fresh and dried American ginseng was different and the content of protein in fresh American ginseng was higher than dried one. The content of vitamin C and vitamin E, SOD activity assay in fresh American ginseng were significantly higher than dried one (P<0.01). The content and the kind of components of volatile oil were different and in fresh American ginseng was more than dried one. Conclusion: The component of protein, vitamin C, vitamin E, volatile oil, SOD activity in fresh American ginseng and dried one was different, which may be one of the reasons for the different of properties and efficacy.